Open AccessOptimization and Technological Development Strategies of an Antimicrobial Extract from Achyrocline alata Assisted by Statistical Design
Author(s) -
Daniel P. Demarque,
Sonia Maria Fernandes Fitts,
Amanda Galdi Boaretto,
Júlio César Leite da Silva,
Maria do Carmo Vieira,
Vanessa Franco,
Caroline Benites Teixeira,
Mônica Cristina Toffoli-Kadri,
Carlos Alexandre Carollo
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0118574
Subject(s) - streptococcus mutans , antimicrobial , response surface methodology , traditional medicine , extraction (chemistry) , food science , chemistry , bacteria , microbiology and biotechnology , biology , chromatography , medicine , genetics
Achyrocline alata , known as Jateí-ka-há, is traditionally used to treat several health problems, including inflammations and infections. This study aimed to optimize an active extract against Streptococcus mutans , the main bacteria that causes caries. The extract was developed using an accelerated solvent extraction and chemometric calculations. Factorial design and response surface methodologies were used to determine the most important variables, such as active compound selectivity. The standardized extraction recovered 99% of the four main compounds, gnaphaliin, helipyrone, obtusifolin and lepidissipyrone, which represent 44% of the extract. The optimized extract of A . alata has a MIC of 62.5 μg/mL against S . mutans and could be used in mouth care products.
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