Correction: Mice Lacking GD3 Synthase Display Morphological Abnormalities in the Sciatic Nerve and Neuronal Disturbances during Peripheral Nerve Regeneration

There are a number of errors in the legends for Figs. 3, 4, and 5. The complete, correct legends for Figs. 3, 4, and 5 are: Figure 3. Wallerian degeneration in mice lacking GD3s: tissue infiltration by activated macrophages and Schwann cell proliferation. A-B; D-E: Longitudinal sections of lesioned sciatic nerves from adult WT and GD3s KO mice at 5 days after crush lesioning immunolabeled for NF-200 (A, B) or F4–80 (D, E) and imaged at the distal nerve stump. The nuclei were counterstained with DAPI. C and F: Histograms indicating the number of NF-200 fragments (C) and active macrophages (cells positive for F4–80) at the distal nerve stump (F). G-H; J-K: Longitudinal sections of lesioned sciatic nerves fromWT and GD3s KO mice imaged at the distal stump 7 days after crush lesioning and immunolabeled for p-histone H3 (G-H) or double immunolabeled for Ki-67 and GFAP (J-K). The nuclei were counterstained with DAPI. I and L: Histograms indicating the number of cells positive for phistone H3 (I) or KI-67/GFAP (L) at the distal stump in each group of mice. M-O: Optical slices obtained by confocal microscopy from transversal sections of wildtype uninjured (M), wildtype injured (N) or GD3s Ko injured (O) sciatic nerves immunolabeled for GFAP at distal stump, 5 days after crush lesion. Bars: A-B, G-H = 100 mm; and D-E, J-K = 50 mm; M-O = 20 mm. Statistics:Mann-Whitney, ns, p>0.05. doi: 10.1371/journal.pone.0108919.g003 Figure 4. Committed nerve regeneration in adult mice lacking GD3s is restored by administration of exogenous GD3 in vivo and in vitro. A: Longitudinal sections of sciatic nerves proximally or 1 mm or 3 mm distally immunolabeled for GAP-43 at 21 days after crush lesioning. B: Histogram indicating the axonal density in the regenerating nerves fromWT, GD3s KO and GD3-treated GD3s KO mice. C: Images of P1 mouse DRG explants seeded on PDL/laminin coverslips. The DRG samples fromWT, GD3s KO and GD3s KO exogenously treated with GD3 ganglioside were incubated for 5 days in vitro. GD3 was added on day 2 of the incubation. Low-magnification images of DRGs immunolabeled for Tuj-1. D: Histogram quantifying neurite growth. E: High-magnification images of neurites immunolabeled for R24 (GD3, O-Q) or CD-60b (9-O-Acetyl GD3, R-T). The nuclei were counterstained with DAPI (white) Bars: A = 100 mm; C = 500 mm; and E = 20 mm. Statistics: ANOVA p<0.001; p<0.01. doi: 10.1371/journal.pone.0108919.g004 Figure 5. Integrin-β1 expression but not calcium influx is modified in neurons lacking GD3s. A-C: Images of integrin-β1 obtained by apotome microscopy of mice neonate (P1) DRGs fromWT (A), GD3s KO (B) and GD3s KO with exogenous GD3 (C). Samples were cultured for 5 days. A0–C0: High-magnification optical sections of neurites (DIC) labeled for integrin-β1. A@-C@: High-magnification optical sections of neurites (DIC) double labeled for