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We here describe a convenient method for preparation, fixation and fluorescence analysis of  in vitro  cultivated metacestode vesicles from  E. multilocularis . Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining assays directly. Using these preparations, in combination with conventional fluorescence staining techniques, we could detect the expression and subcellular localization of a specific protein and identify  in situ  proliferative or apoptotic cells in the germinal layer of metacestode vesicles. Based on this approach, future molecular and cellular analysis of  Echinococcus  metacestode vesicles in the  in vitro  system will be greatly facilitated.

A Rapid and Convenient Method for Fluorescence Analysis of In Vitro Cultivated Metacestode Vesicles from Echinococcus multilocularis