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Emergence of methicillin-resistant  Staphylococcus aureus  (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) in bovine milk is a major public health concern. The primary purpose of this research was to determine molecular genetic characteristics and antibiotic resistance of staphylococcal isolates recovered from milk of mastitic cows in the Shaanxi Province in Northwestern China. One hundred and thirteen methicillin-susceptible  Staphylococcus aureus  (MSSA), one  mecA -positive and phenotype-positive MRSA, seven  mecA - and  mecC - negative but phenotype-positive MRSA and two MR-CoNS including one oxacillin-susceptible  mecA -positive  Staphylococcus haemolyticus  (OS-MRSH) and one  mecA -positive and methicillin-resistant  Staphylococcus epidermidis  (MRSE) isolates were recovered from 214 quarter milk samples on 4 dairy farms. All above 123 isolates were subjected to antibiotic resistance profiling.  S .  aureus  isolates were also genotyped using the  spa  typing and the multilocus sequence typing (MLST). Eight MRSA and 2 MR-CoNS isolates were additionally tested for SCC mec  types. Resistance was common among isolates against ampicillin or penicillin (80.5%), kanamycin (68.3%), gentamicin (67.5%), tetracycline (43.9%) and chloramphenicol (30.1%). However, no isolate was resistant to vancomycin or teicoplanin. Twenty, 29 and 58 isolates showed resistance to 1, 2 or more than 2 antibiotics, respectively. The predominant multidrug resistance profile was penicillin/ampicillin/kanamycin/gentamicin/tetracycline (46 isolates). Most  S .  aureus  isolates belonged to  spa  types t524 (n = 63), t11772 (a new type, n = 31) and t4207 (n = 15). At the same time, MLST types ST71 (n = 67) and ST2738 (a new type, n = 45) were identified as dominant sequence types. The  mecA -positive and phenotype-positive MRSA isolate had a composite genotype t524-ST71-SCC mec IVa, while 7  mecA -negative but phenotype-positive MRSA isolates were all t524-ST71. The OS-MRSH isolate contained a type V SCC mec  cassette, while the MRSE isolate possessed a non-typeable SCC mec . The  spa -MLST types t11772-ST2738 (n = 27), t11807-ST2683 (n = 4) and t11771-ST2738 (n = 3) were newly identified genotypes of  S .  aureus . These new genotypes and multidrug-resistant staphylococci could pose additional threat to animal and human health.

Characterization of Methicillin-Resistant and -Susceptible Staphylococcal Isolates from Bovine Milk in Northwestern China