English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Chaperone/usher (CU) assembly pathway is used by a wide range of  Enterobacteriaceae  to assemble adhesive surface structures called pili or fimbriae that play a role in bacteria-host cell interactions.  In silico  analysis revealed that the genome of  Klebsiella pneumoniae  LM21 harbors eight chromosomal CU loci belonging to γκп and ϭ clusters. Of these, only two correspond to previously described operons, namely type 1 and type 3-encoding operons. Isogenic usher deletion mutants of  K .  pneumoniae  LM21 were constructed for each locus and their role in adhesion to animal (Intestine 407) and plant ( Arabidopsis thaliana ) cells, biofilm formation and murine intestinal colonization was investigated. Type 3 pili usher deleted mutant was impaired in all assays, whereas type 1 pili usher deleted mutant only showed attenuation in adhesion to plant cells and in intestinal colonization. The LM21Δ kpjC  mutant was impaired in its capacity to adhere to  Arabidopsis  cells and to colonize the murine intestine, either alone or in co-inoculation experiments. Deletion of LM21 kpgC  induced a significant decrease in biofilm formation, in adhesion to animal cells and in colonization of the mice intestine. The LM21∆ kpaC  and LM21∆ kpeC  mutants were only attenuated in biofilm formation and the adhesion abilities to  Arabidopsis  cells, respectively. No clear  in vitro  or  in vivo  effect was observed for LM21∆ kpbC  and LM21∆ kpdC  mutants. The multiplicity of CU loci in  K .  pneumoniae  genome and their specific adhesion pattern probably reflect the ability of the bacteria to adhere to different substrates in its diverse ecological niches.

In Silico Analysis of Usher Encoding Genes in Klebsiella pneumoniae and Characterization of Their Role in Adhesion and Colonization