A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies | Zendy

Qi Wang | Zendy; Xiaofei Li | Zendy; Xiaolin Ji | Zendy; Jingfei Wang | Zendy; Nan Shen | Zendy; Yulong Gao | Zendy; Xiaole Qi | Zendy; Yongqiang Wang | Zendy; Honglei Gao | Zendy; Shide Zhang | Zendy; Xiaomei Wang | Zendy

Open Access

A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies

Author(s) -

Qi Wang,

Xiaofei Li,

Xiaolin Ji,

Jingfei Wang,

Nan Shen,

Yulong Gao,

Xiaole Qi,

Yongqiang Wang,

Honglei Gao,

Shide Zhang,

Xiaomei Wang

Publication year - 2014

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0115422

Subject(s) - virology , virus , recombinant dna , outbreak , antibody , biology , neutralization , neutralizing antibody , avian leukosis , recombinant virus , gene , immunology , genetics

Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test.

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