English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Biogenesis and recycling of iron–sulfur (Fe–S) clusters play important roles in the iron homeostasis mechanisms involved in mitochondrial function. In  Saccharomyces cerevisiae , the Fe–S clusters are assembled into apoproteins by the iron–sulfur cluster machinery (ISC). The aim of the present study was to determine the effects of ISC gene deletion and consequent iron release under oxidative stress conditions on mitochondrial functionality in  S. cerevisiae . Reactive oxygen species (ROS) generation, caused by H 2 O 2 , menadione, or ethanol, was associated with a loss of iron homeostasis and exacerbated by ISC system dysfunction.  ISC  mutants showed increased free Fe 2+  content, exacerbated by ROS-inducers, causing an increase in ROS, which was decreased by the addition of an iron chelator. Our study suggests that the increment in free Fe 2+  associated with ROS generation may have originated from mitochondria, probably Fe–S cluster proteins, under both normal and oxidative stress conditions, suggesting that Fe–S cluster anabolism is affected. Raman spectroscopy analysis and immunoblotting indicated that in mitochondria from  SSQ1  and  ISA1  mutants, the content of [Fe–S] centers was decreased, as was formation of Rieske protein-dependent supercomplex III 2 IV 2 , but this was not observed in the iron-deficient  ATX1  and  MRS4  mutants. In addition, the activity of complexes II and IV from the electron transport chain (ETC) was impaired or totally abolished in  SSQ1  and  ISA1  mutants. These results confirm that the ISC system plays important roles in iron homeostasis, ROS stress, and in assembly of supercomplexes III 2 IV 2  and III 2 IV 1 , thus affecting the functionality of the respiratory chain.

plos one

Malfunctioning of the Iron–Sulfur Cluster Assembly Machinery in Saccharomyces cerevisiae Produces Oxidative Stress via an Iron-Dependent Mechanism, Causing Dysfunction in Respiratory Complexes