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The µ opioid receptor gene,  OPRM1 , undergoes extensive alternative pre-mRNA splicing in rodents and humans, with dozens of alternatively spliced variants of the  OPRM1  gene. The present studies establish a SYBR green quantitative PCR (qPCR) assay to more accurately quantify mouse  OPRM1  splice variant mRNAs. Using these qPCR assays, we examined the expression of  OPRM1  splice variant mRNAs in selected brain regions of four inbred mouse strains displaying differences in µ opioid-induced tolerance and physical dependence: C56BL/6J, 129P3/J, SJL/J and SWR/J. The complete mRNA expression profiles of the  OPRM1  splice variants reveal marked differences of the variant mRNA expression among the brain regions in each mouse strain, suggesting region-specific alternative splicing of the  OPRM1  gene. The expression of many variants was also strain-specific, implying a genetic influence on  OPRM1  alternative splicing. The expression levels of a number of the variant mRNAs in certain brain regions appear to correlate with strain sensitivities to morphine analgesia, tolerance and physical dependence in four mouse strains.

plos one

Differential Expressions of the Alternatively Spliced Variant mRNAs of the µ Opioid Receptor Gene, OPRM1, in Brain Regions of Four Inbred Mouse Strains