Peroxidase-Like Catalytic Activity of Ag3PO4 Nanocrystals Prepared by a Colloidal Route

Nearly monodispersed Ag 3 PO 4 nanocrystals with size of 10 nm were prepared through a colloidal chemical route. It was proven that the synthesized Ag 3 PO 4 nanoparticles have intrinsic peroxidase-like catalytic activity. They can quickly catalyze oxidation of the peroxidase substrate 3, 3, 5, 5-tetramethylbenzidine (TMB) in the presence of H 2 O 2 , producing a blue color. The catalysis reaction follows Michaelis-Menten kinetics. The calculated kinetic parameters indicate a high catalytic activity and the strong affinity of Ag 3 PO 4 nanocrystals to the substrate (TMB). These results suggest the potential applications of Ag 3 PO 4 nanocrystals in fields such as biotechnology, environmental chemistry, and medicine.