English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background    Stenotrophomonas maltophilia  is emerging as one of the most frequently found bacteria in chronic pulmonary infection. Biofilm is increasingly recognized as a contributing factor to disease pathogenesis. In the present study, a total of 37 isolates of  S. maltophilia  obtained from chronic pulmonary infection patients were evaluated to the relationship between biofilm production and the relative genes expression.    Methods  The clonal relatedness of isolates was determined by pulse-field gel electrophoresis. Biofilm formation assays were performed by crystal violet assay, and confirmed by Electron microscopy analysis and CLSM analysis. PCR was employed to learn gene distribution and expression.    Results  Twenty-four pulsotypes were designated for 37  S. maltophilia  isolates, and these 24 pulsotypes exhibited various levels of biofilm production, 8 strong biofilm-producing  S. maltophilia  strains with OD492 value above 0.6, 14 middle biofilm-producing strains with OD492 average value of 0.4 and 2 weak biofilm-producing strains with OD492 average value of 0.19. CLSM analysis showed that the isolates from the early stage of chronic infection enable to form more highly structured and multilayered biofim than those in the late stage. The prevalence of  spgM ,  rmlA , and  rpfF  genes was 83.3%, 87.5%, and 50.0% in 24  S. maltophilia  strains, respectively, and the presence of  rmlA ,  spgM  or  rpfF  had a close relationship with biofilm formation but did not significantly affect the mean amount of biofilm. Significant mutations of  spgM  and  rmlA  were found in both strong and weak biofilm-producing strains.    Conclusion  Mutations in  spgM  and  rmlA  may be relevant to biofilm formation in the clinical isolates of  S. maltophilia .

The Impact of spgM, rpfF, rmlA Gene Distribution on Biofilm Formation in Stenotrophomonas maltophilia