Correction: Brain Region-Specific Expression of MeCP2 Isoforms Correlates with DNA Methylation within Mecp2 Regulatory Elements

The Figure panel 2C (loading control), Supplementary Figure panels S1A (loading control) and S3A (upper panel) reported in Olson et al., 2014 [1] are incorrect. The GADPH loading control in Figure 2C is the mirror image of the ACTIN loading control in Figure 1C of a previous publication [2], and the upper panel of Figure S3A is the same as the upper panel of Figure 1A in the current paper. The GAPDH loading control in Figure S1A is a processed image (2u counter clockwise rotation) from the loading control of a Western blot, from which lanes 3 and 4 correspond to the loading control in Figure 2G in the current paper. The authors apologize for these errors, which happened during the assembly of the figures. Please refer to the corrected Figure 2 here, and the corrected Figures S1 and S3 in the Supporting Information section of this correction. The raw data corresponding to each corrected figure panel is also included. These corrections do not affect the results or conclusions reported in the article. The authors also correct the following statement in the Introduction: Paragraph 2 on page 2: ‘‘Due to the lack of anti-MeCP2E2 antibodies, comparative analysis of both MeCP2 isoforms at the protein levels in any system has not been reported to date.’’ While this manuscript was under review, an article was published by Yasui et al. in Human Molecular Genetics, which did report MeCP2E1 and E2 at the protein level in mouse brain [3]. This study was cited as reference 22 but the statement in the Introduction should have been revised to reflect the findings by Yasui et al. In addition, a publication by Kaddoum et al. reported an analysis of expression of the MeCP2 isoforms in mouse brain [4]. The authors would like to revise the statement in the Introduction to read as below: ‘‘Dr. Dag Yasui’s group, in collaboration with our lab, has recently reported the expression of MeCP2E1 and MeCP2E2 in the mouse brain [22]. Kaddoum et al. also reported an expression analysis of the MeCP2 isoforms in mouse brain. However, a comparative transcript and protein correlation analysis of the two Mecp2/MeCP2 isoforms in different brain regions and during mouse brain development has not been reported previously.’’