Open AccessHuman Papillomavirus 16 Non-European Variants Are Preferentially Associated with High-Grade Cervical Lesions
Author(s) -
Luciana Bueno de Freitas,
Zigui Chen,
Elaine Freire Muqui,
Neide Aparecida Tosato Boldrini,
Angélica Espinosa Miranda,
Liliana Cruz Spano,
Robert D. Burk
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0100746
Subject(s) - colposcopy , cervical cancer , cervical intraepithelial neoplasia , phylogenetic tree , medicine , cytology , biology , genotype , cancer , genetics , gene
HPV16 accounts for 50–70% of cervical cancer cases worldwide. Characterization of HPV16 variants previously indicated that they differ in risks for viral persistence, progression to cervical precancer and malignant cancer. The aim of this study was to examine the association of severity of disease with HPV16 variants identified in specimens (n = 281) obtained from a Cervical Pathology and Colposcopy outpatient clinic in the University Hospital of Espírito Santo State, Southeastern Brazil, from April 2010 to November 2011. All cytologic and histologic diagnoses were determined prior to definitive treatment. The DNA was isolated using QIAamp DNA Mini Kit and HPV was detected by amplification with PGMY09/11 primers and positive samples were genotyped by RFLP analyses and reverse line blot. The genomes of the HPV16 positive samples were sequenced, from which variant lineages were determined. Chi 2 statistics was performed to test the association of HPV16 variants between case and control groups. The prevalence of HR-HPV types in
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