English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Coding sequences for major trichome regulatory genes, including the positive regulators  GLABRA 1 ( GL1 ),  GLABRA 2  ( GL2 ),  ENHANCER OF GLABRA 3  ( EGL3 ), and  TRANSPARENT TESTA GLABRA  1 ( TTG1 ) and the negative regulator  TRIPTYCHON  ( TRY ), were cloned from wild  Brassica villosa , which is characterized by dense trichome coverage over most of the plant. Transcript (FPKM) levels from RNA sequencing indicated much higher expression of the  GL2  and  TTG1  regulatory genes in  B. villosa  leaves compared with expression levels of  GL1  and  EGL3  genes in either  B. villosa  or the reference genome species, glabrous  B. oleracea ; however, cotyledon  TTG1  expression was high in both species. RNA sequencing and Q-PCR also revealed an unusual expression pattern for the negative regulators  TRY  and CPC, which were much more highly expressed in trichome-rich  B. villosa  leaves than in glabrous  B. oleracea  leaves and in glabrous cotyledons from both species. The  B. villosa  TRY expression pattern also contrasted with TRY expression patterns in two diploid Brassica species, and with the Arabidopsis model for expression of negative regulators of trichome development. Further unique sequence polymorphisms, protein characteristics, and gene evolution studies highlighted specific amino acids in  GL1  and  GL2  coding sequences that distinguished glabrous species from hairy species and several variants that were specific for each  B. villosa  gene. Positive selection was observed for GL1 between hairy and non-hairy plants, and as expected the origin of the four expressed positive trichome regulatory genes in  B. villosa  was predicted to be from  B. oleracea . In particular the unpredicted expression patterns for  TRY  and  CPC  in  B. villosa  suggest additional characterization is needed to determine the function of the expanded families of trichome regulatory genes in more complex polyploid species within the  Brassicaceae .

Comparison of Five Major Trichome Regulatory Genes in Brassica villosa with Orthologues within the Brassicaceae