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A challenge to conducting high-impact and reproducible studies of the mechanisms of  P. falciparum  drug resistance, invasion, virulence, and immunity is the lack of robust and sustainable  in vitro  culture in the field. While the technology exists and is routinely utilized in developed countries, various factors–from cost, to supply, to quality–make it hard to implement in malaria endemic countries. Here, we design and rigorously evaluate an adjustable gas-mixing device for the  in vitro  culture of  P. falciparum  parasites in the field to circumvent this challenge. The device accurately replicates the gas concentrations needed to culture laboratory isolates, short-term adapted field isolates, cryopreserved previously non-adapted isolates, as well as to adapt  ex vivo  isolates to  in vitro  culture in the field. We also show an advantage over existing alternatives both in cost and in supply. Furthermore, the adjustable nature of the device makes it an ideal tool for many applications in which varied gas concentrations could be critical to culture success. This adjustable gas-mixing device will dramatically improve the feasibility of  in vitro  culture of  Plasmodium falciparum  parasites in malaria endemic countries given its numerous advantages.

An Adjustable Gas-Mixing Device to Increase Feasibility of In Vitro Culture of Plasmodium falciparum Parasites in the Field