Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis | Zendy

Athanasios Niarchos | Zendy; Marios Zouridakis | Zendy; Vassilis Douris | Zendy; Assimina Georgostathi | Zendy; Dimitra Kalamida | Zendy; Alexandros Sotiriadis | Zendy; Konstantinos Poulas | Zendy; Kostas Iatrou | Zendy; Socrates J. Tzartos | Zendy

Open Access

Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis

Author(s) -

Athanasios Niarchos,

Marios Zouridakis,

Vassilis Douris,

Assimina Georgostathi,

Dimitra Kalamida,

Alexandros Sotiriadis,

Konstantinos Poulas,

Kostas Iatrou,

Socrates J. Tzartos

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0084791

Subject(s) - nicotinic acetylcholine receptor , acetylcholine receptor , myasthenia gravis , antigen , autoantibody , acetylcholine , epitope , extracellular , monoclonal antibody , microbiology and biotechnology , receptor , chemistry , antibody , biology , immunology , biochemistry , pharmacology

We describe the expression of the extracellular domain of the human α1 nicotinic acetylcholine receptor (nAChR) in lepidopteran insect cells (i-α1-ECD) and its suitability for use in antigen-specific therapies for Myasthenia Gravis (MG). Compared to the previously expressed protein in P. pastoris (y-α1-ECD), i - α1 - ECD had a 2-fold increased expression yield, bound anti-nAChR monoclonal antibodies and autoantibodies from MG patients two to several-fold more efficiently and resulted in a secondary structure closer to that of the crystal structure of mouse α1-ECD. Our results indicate that i - α1 - ECD is an improved protein for use in antigen-specific MG therapeutic strategies.

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