Determination of HER2 Amplification Status on Tumour DNA by Digital PCR | Zendy

Isaac García-Murillas | Zendy; Maryou Lambros | Zendy; Nicholas C. Turner | Zendy

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Determination of HER2 Amplification Status on Tumour DNA by Digital PCR

Author(s) -

Isaac García-Murillas,

Maryou Lambros,

Nicholas C. Turner

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0083409

Subject(s) - digital polymerase chain reaction , microbiology and biotechnology , concordance , biology , polymerase chain reaction , dna , real time polymerase chain reaction , genetics , gene

Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2 : EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2:EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2:EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status.

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