A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence | Zendy

Randy F. Stout | Zendy; Vladimir Grubišić | Zendy; Vladimir Parpura | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence

Author(s) -

Randy F. Stout,

Vladimir Grubišić,

Vladimir Parpura

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0081771

Subject(s) - caenorhabditis elegans , transgene , biology , phenotype , gene , genetics , genetically modified mouse , promoter , coding region , caenorhabditis , microbiology and biotechnology , gene expression

Transgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding transgenic sequence produced profound changes to the behavior of the nematode Caenorhabditis elegans . These changes were produced by a glial promoter sequence but, unexpectedly, major deficits were observed specifically in backward locomotion, a neuron-driven behavior. We also present evidence that this behavioral phenotype is transpromoter copy number-dependent and manifests early in development and is maintained into adulthood of the worm.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research