Open AccessA Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C
Author(s) -
Camila da Silva Ferreira,
Rodrigo Martins Abreu,
Marlone CunhaSilva,
Aline Siqueira Ferreira,
Paulo Dominguez Nasser,
Flair José Carrilho,
Ono S
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0078142
Subject(s) - interleukin 28b , pegylated interferon , ribavirin , multiplex polymerase chain reaction , virology , polymerase chain reaction , hepatitis c , multiplex , hepatitis c virus , biology , single nucleotide polymorphism , interferon , genotype , medicine , gene , virus , genetics
Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods.
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