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Core components of the secretory pathway have largely been identified and studied in single cell systems such as the budding yeast  S. cerevisiae  or in mammalian tissue culture. These studies provide details on the molecular functions of the secretory machinery; they fail, however, to provide insight into the role of these proteins in the context of specialized organs of higher eukaryotes. Here, we identify and characterize the first loss-of-function mutations in a KDEL receptor gene from higher eukaryotes. Transcripts from the Drosophila KDEL receptor gene  KdelR  – formerly known as  dmErd2  – are provided maternally and, at later stages, are at elevated levels in several embryonic cell types, including the salivary gland secretory cells, the fat body and the epidermis. We show that, unlike  Saccharomyces cerevisiae Erd2  mutants, which are viable,  KdelR  mutations are early larval lethal, with homozygous mutant animals dying as first instar larvae.  KdelR  mutants have larval cuticle defects similar to those observed with loss-of-function mutations in other core secretory pathway genes and with mutations in  CrebA , which encodes a bZip transcription factor that coordinately upregulates secretory pathway component genes in specialized secretory cell types. Using the salivary gland, we demonstrate a requirement for  KdelR  in maintaining the ER pool of a subset of soluble resident ER proteins. These studies underscore the utility of the Drosophila salivary gland as a unique system for studying the molecular machinery of the secretory pathway  in vivo  in a complex eukaryote.

Drosophila KDEL Receptor Function in the Embryonic Salivary Gland and Epidermis