Open AccessInfluence of DNMT Genotype on Global and Site Specific DNA Methylation Patterns in Neonates and Pregnant Women
Author(s) -
Catherine Potter,
Jill A. McKay,
Alexandra Groom,
Dianne Ford,
Lisa Coneyworth,
John C. Mathers,
Caroline L. Relton
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0076506
Subject(s) - dna methylation , methylation , genotype , biology , genetics , dna methyltransferase , pyrosequencing , epigenetics , dnmt1 , genetic variation , gene , methyltransferase , microbiology and biotechnology , gene expression
This study examines the relationship between common genetic variation within DNA methyltransferase genes and inter-individual variation in DNA methylation. Eleven polymorphisms spanning DNMT1 and DNMT3B were genotyped. Global and gene specific ( IGF2, IGFBP3, ZNT5 ) DNA methylation was quantified by LUMA and bisulfite Pyrosequencing assays, respectively, in neonatal cord blood and in maternal peripheral blood. Associations between maternal genotype and maternal methylation (n ≈ 333), neonatal genotype and neonatal methylation (n ≈ 454), and maternal genotype and neonatal methylation (n ≈ 137) were assessed. The findings of this study provide some support to the hypothesis that genetic variation in DNA methylating enzymes influence DNA methylation at global and gene-specific levels; however observations were not robust to correction for multiple testing. More comprehensive analysis of the influence of genetic variation on global and site specific DNA methylation is warranted.
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