English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Geobacillus stearothermophilus  is a gram-positive, thermophilic bacterium, spores of which are very heat resistant. Raman spectroscopy and differential interference contrast microscopy were used to monitor the kinetics of germination of individual spores of  G. stearothermophilus  at different temperatures, and major conclusions from this work were as follows. 1) The CaDPA level of individual  G. stearothermophilus  spores was similar to that of  Bacillus  spores. However, the Raman spectra of protein amide bands suggested there are differences in protein structure in spores of  G. stearothermophilus  and  Bacillus  species. 2) During nutrient germination of  G. stearothermophilus  spores, CaDPA was released beginning after a lag time ( T   lag ) between addition of nutrient germinants and initiation of CaDPA release. CaDPA release was complete at  T   release , and Δ T   release  ( T   release  –  T   lag ) was 1–2 min. 3) Activation by heat or sodium nitrite was essential for efficient nutrient germination of  G. stearothermophilus  spores, primarily by decreasing  T   lag  values. 4) Values of  T   lag  and  T   release  were heterogeneous among individual spores, but Δ T   release  values were relatively constant. 5) Temperature had major effects on nutrient germination of  G. stearothermophilus  spores, as at temperatures below 65°C, average  T   lag  values increased significantly. 6)  G. stearothermophilus  spore germination with exogenous CaDPA or dodecylamine was fastest at 65°C, with longer T lag  values at lower temperatures. 7) Decoating of  G. stearothermophilus  spores slowed nutrient germination slightly and CaDPA germination significantly, but increased dodecylamine germination markedly. These results indicate that the dynamics and heterogeneity of the germination of individual  G. stearothermophilus  spores are generally similar to that of  Bacillus  species.

plos one

Kinetics of Germination of Individual Spores of Geobacillus stearothermophilus as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy