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Methicillin-resistant  Staphylococcus aureus  (MRSA) ST398, associated with livestock animals, was described in 2003 as a new lineage infecting or colonizing humans. We evaluated the prevalence and molecular characteristics of MRSA ST398 isolated in the Hospital Universitari de Bellvitge from January 2000 to June 2011. Tetracycline resistant (Tet-R) MRSA isolates from single patients (pts) were screened by  Sma I-pulsed field gel electrophoresis (PFGE). Nontypable MRSA strains by  Sma I (NT  Sma    I )-MRSA were further analysed by  Apa I-PFGE,  spa , SCC mec, agr , MLST typing, and by DNA microarray hybridization. Among 164 pts harboring Tet-R MRSA, NT  Sma    I -MRSA ST398- agr I was found in 33 pts (20%). Although the first pt was detected in 2003, 22/33 pts (67%) were registered in the 2010–2011 period. Ten pts (30%) were infected and cancer was the most frequent underlying disease. In one case, death was due to MRSA-ST398-related infection. Five pulsotypes (A–E) were detected using  Apa I-PFGE, with type A accounting for 76% of the strains. The majority of the studied isolates presented  spa  type t011 (70%) and SCC mec  type V (88%). One strain was  spa  negative both by PCR and microarray analysis. Forty-nine percent of the studied isolates showed resistance to 3 or more antibiotic classes, in addition to beta-lactams. Ciprofloxacin resistance was 67%. Tet-R was mediated by  tet (M) and  tet (K) in 26 isolates. All isolates lacked Panton-Valentine Leukocidin production, as well as other significant toxins. This study displays the molecular features of MRSA-ST398 clone and shows the increase in tetracycline resistance together with arise in MRSA-ST398 isolates infecting or colonizing patients in our clinical setting.

plos one

Prevalence and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus ST398 Resistant to Tetracycline at a Spanish Hospital over 12 Years