Pituitary Adenylate Cyclase Activating Peptide (PACAP) Participates in Adipogenesis by Activating ERK Signaling Pathway | Zendy

Tatjana Arsenijevic | Zendy; Françoise Grégoire | Zendy; Jeanne Chiadak | Zendy; Elodie Courtequisse | Zendy; Nargis Bolaky | Zendy; Jason Perret | Zendy; Christine Delporte | Zendy

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Pituitary Adenylate Cyclase Activating Peptide (PACAP) Participates in Adipogenesis by Activating ERK Signaling Pathway

Author(s) -

Tatjana Arsenijevic,

Françoise Grégoire,

Jeanne Chiadak,

Elodie Courtequisse,

Nargis Bolaky,

Jason Perret,

Christine Delporte

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0072607

Subject(s) - vasoactive intestinal peptide , adenylate kinase , receptor , cyclase , pituitary adenylate cyclase activating peptide , secretin , mapk/erk pathway , adipogenesis , endocrinology , chemistry , phosphorylation , medicine , biology , microbiology and biotechnology , neuropeptide , biochemistry , adipose tissue , secretion

Pituitary adenylate cyclase activating peptide (PACAP) belongs to the secretin/glucagon/vasoactive intestinal peptide (VIP) family. Its action can be mediated by three different receptor subtypes: PAC1, which has exclusive affinity for PACAP, and VPAC1 and VPAC2 which have equal affinity for PACAP and VIP. We showed that all three receptors are expressed in 3T3-L1 cells throughout their differentiation into adipocytes. We established the activity of these receptors by cAMP accumulation upon induction by PACAP. Together with insulin and dexamethasone, PACAP induced adipogenesis in 3T3-L1 cell line. PACAP increased cAMP production within 15 min upon stimulation and targeted the expression and phosphorylation of MAPK (ERK1/2), strengthened by the ERK1/2 phosphorylation being partially or completely abolished by different combinations of PACAP receptors antagonists. We therefore speculate that ERK1/2 activation is crucial for the activation of CCAAT/enhancer- binding protein β (C/EBPβ).

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