Open AccessMurine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
Author(s) -
Emili Cid,
Miyako Yamamoto,
Marcus Buschbeck,
Fumiichiro Yamamoto
Publication year - 2013
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0064728
Subject(s) - glycolipid , glycosyltransferase , microbiology and biotechnology , embryonic stem cell , cell , cell sorting , biology , computational biology , chemistry , gene , biochemistry
Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins.
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