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Plant-parasitic nematodes produce at least one structurally unique class of small helix-rich retinol- and fatty-acid-binding proteins that have no counterparts in their plant hosts. Herein we describe a protein of the plant-parasitic root-knot nematode  Meloidogyne javanica , which is a member of the nematode-specific fatty-acid- and retinol-binding (Mj-FAR-1) family of proteins. The  mj-far - 1  mRNA was detected through  M. javanica  pre-parasitic J2s, migratory and sedentary parasitic stages by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Immunolocalization assays demonstrate that the FAR protein of  Meloidogyne  is secreted during sedentary stages, as evidenced by the accumulation of FAR at the nematode cuticle surface and along the adjacent host root tissues. Tomato roots constitutively expressing  mj-far - 1  demonstrated an increased susceptibility to root-knot nematodes infection as observed by accelerated gall induction and expansion, accompanied by a higher percentage of nematodes developing into mature females compared to control roots. RNA interference assays that expressed double-stranded RNA complementary to  mj-far-1  in transgenic tomato lines specifically reduced nematode infection levels. Histological analysis of nematode-infested roots indicated that in roots overexpressing  mj-far - 1 , galls contained larger feeding cells and might support a faster nematode development and maturation. Roots overexpressing  mj-far - 1  suppressed jasmonic acid responsive genes such as the proteinase inhibitor ( Pin2 ) and γ -thionin , illustrating the possible role of Mj-FAR-1 in manipulating the lipid based signaling  in planta . This data, suggests that  Meloidogyne  FAR might have a strategic function during the interaction of the nematode with its plant host. Our study present the first demonstration of an  in planta  functional characterization and localization of FAR proteins secreted by plant-parasitic nematodes. It provides evidence that Mj-FAR-1 facilitates infection most likely via the manipulation of host lipid-based defenses, as critical components for a successful parasitism by plant-parasitic nematodes.

Fatty Acid-and Retinol-Binding Protein, Mj-FAR-1 Induces Tomato Host Susceptibility to Root-Knot Nematodes