Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid | Zendy

Kunihiro Kaihatsu | Zendy; Shinjiro Sawada | Zendy; Shota Nakamura | Zendy; Takaaki Nakaya | Zendy; Teruo Yasunaga | Zendy; Nobuo Kato | Zendy

Open Access

Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid

Author(s) -

Kunihiro Kaihatsu,

Shinjiro Sawada,

Shota Nakamura,

Takaaki Nakaya,

Teruo Yasunaga,

Nobuo Kato

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0064017

Subject(s) - virus , biology , influenza a virus , virology , nucleic acid , ribonucleoprotein , peptide sequence , rna , gene , genome , sequence analysis , genetics

To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.

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