Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment | Zendy

Jon Celay | Zendy; Idoia BlancoLuquin | Zendy; Paula Lázcoz | Zendy; Mirja Rotinen | Zendy; Javier S. Castresana | Zendy; Ignacio Encı́o | Zendy

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Changes in Gene Expression Profiling of Apoptotic Genes in Neuroblastoma Cell Lines upon Retinoic Acid Treatment

Author(s) -

Jon Celay,

Idoia BlancoLuquin,

Paula Lázcoz,

Mirja Rotinen,

Javier S. Castresana,

Ignacio Encı́o

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0062771

Subject(s) - retinoic acid , apoptosis , neuroblastoma , flow cytometry , cell culture , microbiology and biotechnology , gene expression profiling , biology , cell , tretinoin , cell growth , gene expression , cancer research , gene , chemistry , biochemistry , genetics

To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9- cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9- cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.

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