A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons | Zendy

Aleksandar Zocevic | Zendy; Fabien Vorimore | Zendy; Nadia Vicari | Zendy; Julien Gasparini | Zendy; Lisa Jacquin | Zendy; Konrad Sachse | Zendy; Simone Magnino | Zendy; Karine Laroucau | Zendy

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A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons

Author(s) -

Aleksandar Zocevic,

Fabien Vorimore,

Nadia Vicari,

Julien Gasparini,

Lisa Jacquin,

Konrad Sachse,

Simone Magnino,

Karine Laroucau

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0058741

Subject(s) - chlamydiaceae , biology , chlamydia psittaci , chlamydophila , chlamydia , chlamydiales , real time polymerase chain reaction , virology , genetics , gene

Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae , requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.

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