Transcriptome Analysis of the Role of GlnD/GlnBK in Nitrogen Stress Adaptation by Sinorhizobium meliloti Rm1021 | Zendy

Svetla. Yurgel | Zendy; Jennifer Rice | Zendy; Michael L. Kahn | Zendy

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Transcriptome Analysis of the Role of GlnD/GlnBK in Nitrogen Stress Adaptation by Sinorhizobium meliloti Rm1021

Author(s) -

Svetla. Yurgel,

Jennifer Rice,

Michael L. Kahn

Publication year - 2013

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0058028

Subject(s) - transcriptome , biology , sinorhizobium meliloti , gene , genome , genetics , microarray analysis techniques , adaptation (eye) , strain (injury) , gene expression , mutant , anatomy , neuroscience

Transcriptional changes in the nitrogen stress response (NSR) of wild type S. meliloti Rm1021, and isogenic strains missing both PII proteins, GlnB and GlnK, or carrying a Δ glnD -sm2 mutation were analyzed using whole-genome microarrays. This approach allowed us to identify a number of new genes involved in the NSR and showed that the response of these bacteria to nitrogen stress overlaps with other stress responses, including induction of the fixK2 transcriptional activator and genes that are part of the phosphate stress response. Our data also show that GlnD and GlnBK proteins may regulate many genes that are not part of the NSR. Analysis of transcriptome profiles of the Rm1021 Δ glnD -sm2 strain allowed us to identify several genes that appear to be regulated by GlnD without the participation of the PII proteins.

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