English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We have previously shown that the member of the HES family  hairy2  induces the ectopic expression of dorsal markers when it is overexpressed in the ventral side of  Xenopus  embryos. Intriguingly,  hairy2  represses the mesoderm transcription factor  brachyury  ( bra ) throughout its domain in the marginal zone. Here we show that in early gastrula,  bra  and  hairy2  are expressed in complementary domains. Overexpression of  bra  repressed  hairy2.  Interference of  bra  function with a dominant-negative construct expanded the  hairy2  domain and, like  hairy2  overexpression, promoted ectopic expression of dorsal axial markers in the ventral side and induced secondary axes without head and notochord.  Hairy2  depletion rescued the ectopic dorsal development induced by interference of  bra  function. We concluded that an intact  bra  function is necessary to exclude  hairy2  expression from the non-organiser field, to impede the ectopic specification of dorsal axial fates and the appearance of incomplete secondary axes. This evidence supports a previously unrecognised role for  bra  in maintaining the dorsal fates inhibited in the ventral marginal zone, preventing the appearance of trunk duplications.

An Intact Brachyury Function Is Necessary to Prevent Spurious Axial Development in Xenopus laevis