English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The brucellae are Gram-negative bacteria that cause an important zoonosis. Studies with the main  Brucella  species have shown that the O-antigens of the  Brucella  smooth lipopolysaccharide are α-(1→2) and α-(1→3)-linked  N -formyl-perosamine polysaccharides that carry M, A and C (A = M, A>M and A<M) epitopes relevant in serodiagnosis and typing. We report that, in contrast to the  B. suis  biovar 1 O-antigen used as a reference or to all described  Brucella  O-antigens,  B. suis  biovar 2 O-antigen failed to bind monoclonal antibodies of C (A = M), C (M>A) and M specificities. However, the biovar 2 O-antigen bound monoclonal antibodies to the  Brucella  A epitope, and to the C/Y epitope shared by brucellae and  Yersinia enterocolitica  O:9, a bacterium that carries an  N -formyl-perosamine O-antigen in exclusively α-(1→2)-linkages. By  13 C NMR spectroscopy,  B. suis  biovar 1 but not  B. suis  biovar 2 or  Y. enterocolitica  O:9 polysaccharide showed the signal characteristic of α-(1→3)-linked  N -formyl-perosamine, indicating that biovar 2 may altogether lack this linkage. Taken together, the NMR spectroscopy and monoclonal antibody analyses strongly suggest a role for α-(1→3)-linked  N -formyl-perosamine in the C (A = M) and C (M>A) epitopes. Moreover, they indicate that  B. suis  biovar 2 O-antigen lacks some lipopolysaccharide epitopes previously thought to be present in all smooth brucellae, thus representing a new brucella serovar that is M-negative, C-negative. Serologically and structurally this new serovar is more similar to  Y. enterocolitica  O:9 than to other brucellae.

plos one

The Epitopic and Structural Characterization of Brucella suis Biovar 2 O-Polysaccharide Demonstrates the Existence of a New M-Negative C-Negative Smooth Brucella Serovar