A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity | Zendy

Tatsuhiko Ozawa | Zendy; Xiuhong Piao | Zendy; Eiji Kobayashi | Zendy; Yue Zhou | Zendy; Hiroaki Sakurai | Zendy; Tsugunobu Andoh | Zendy; Aishun Jin | Zendy; Hiroyuki Kishi | Zendy; Atsushi Muraguchi | Zendy

Open Access

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Author(s) -

Tatsuhiko Ozawa,

Xiuhong Piao,

Eiji Kobayashi,

Yue Zhou,

Hiroaki Sakurai,

Tsugunobu Andoh,

Aishun Jin,

Hiroyuki Kishi,

Atsushi Muraguchi

Publication year - 2012

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0052383

Subject(s) - monoclonal antibody , antigen , antibody , microbiology and biotechnology , rabbit (cipher) , biology , monoclonal , immunology , computer science , computer security

Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system using immunospot array assay on a chip (ISAAC) technology in which we detect and retrieve antigen-specific antibody-secreting cells from the peripheral blood lymphocytes of antigen-immunized rabbits and produce antigen-specific RaMoAbs with 10 –12 M affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.

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