Open AccessOptogenetic Control of Transcription in Zebrafish
Author(s) -
Hongtao Liu,
Gustavo A. Gomez,
Sophia Lin,
Shuo Lin,
Chentao Lin
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0050738
Subject(s) - zebrafish , optogenetics , cryptochrome , transcription factor , biology , transcription (linguistics) , blue light , microbiology and biotechnology , genetics , gene , neuroscience , circadian clock , physics , linguistics , philosophy , optics
Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
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