Open AccessDNA Repair and Cell Cycle Biomarkers of Radiation Exposure and Inflammation Stress in Human Blood
Author(s) -
Helen Budworth,
Antoine M. Snijders,
Francesco Marchetti,
Brandon J. Mannion,
Sandhya Bhatnagar,
Ely Kwoh,
Yuan-De Tan,
Shan X. Wang,
William F. Blakely,
Matthew A. Coleman,
L. E. Peterson,
Andrew J. Wyrobek
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0048619
Subject(s) - dna repair , dna damage , inflammation , ionizing radiation , context (archaeology) , ex vivo , gene expression , gene , in vivo , oxidative stress , biology , lipopolysaccharide , medicine , andrology , immunology , dna , irradiation , genetics , paleontology , physics , nuclear physics
DNA damage and repair are hallmarks of cellular responses to ionizing radiation. We hypothesized that monitoring the expression of DNA repair-associated genes would enhance the detection of individuals exposed to radiation versus other forms of physiological stress. We employed the human blood ex vivo radiation model to investigate the expression responses of DNA repair genes in repeated blood samples from healthy, non-smoking men and women exposed to 2 Gy of X-rays in the context of inflammation stress mimicked by the bacterial endotoxin lipopolysaccharide (LPS). Radiation exposure significantly modulated the transcript expression of 12 genes of 40 tested (2.2E-06
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