Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio) | Zendy

Chia-Hao Lin | Zendy; Che-Hsien Su | Zendy; Deng-Yu Tseng | Zendy; Feng-Chun Ding | Zendy; PungPung Hwang | Zendy

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Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio)

Author(s) -

Chia-Hao Lin,

Che-Hsien Su,

Deng-Yu Tseng,

Feng-Chun Ding,

PungPung Hwang

Publication year - 2012

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0045650

Subject(s) - zebrafish , calcitriol receptor , danio , gene knockdown , calcium , vitamin d and neurology , medicine , endocrinology , biology , cyp24a1 , trpv6 , calcium metabolism , chemistry , microbiology and biotechnology , biochemistry , gene

The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca 2+ uptake function. Low-Ca 2+ fresh water stimulated Ca 2+ influx and expressions of epithelial calcium channel ( ecac ), vitamin D-25-hydroxylase ( cyp2r1 ), vitamin D receptor a ( vdra ), and vdrb in zebrafish. Exogenous vitamin D increased Ca 2+ influx and expressions of ecac and 25-hydroxyvitamin D 3 -24-hydroxylase (cyp24a1) , but downregulated 1α-OHase ( cyp27b1 ) with no effects on other Ca 2+ transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac , and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca 2+ uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca 2+ -handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca 2+ homeostasis and the related physiological processes in vertebrates.

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