Open AccessIsolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells
Author(s) -
SangBum Park,
MinSoo Seo,
HyungSik Kim,
KyungSun Kang
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0044693
Subject(s) - amniotic stem cells , multipotent stem cell , stem cell , mesenchymal stem cell , microbiology and biotechnology , cell therapy , amniotic epithelial cells , population , immunophenotyping , wound healing , adult stem cell , stem cell transplantation for articular cartilage repair , biology , regenerative medicine , flow cytometry , immunology , pathology , cellular differentiation , medicine , progenitor cell , biochemistry , environmental health , gene
Recent studies have shown that amniotic membrane tissue is a rich source of stem cells in humans. In clinical applications, the amniotic membrane tissue had therapeutic effects on wound healing and corneal surface reconstruction. Here, we successfully isolated and identified multipotent stem cells (MSCs) from canine amniotic membrane tissue. We cultured the canine amniotic membrane-derived multipotent stem cells (cAM-MSCs) in low glucose DMEM medium. cAM-MSCs have a fibroblast-like shape and adhere to tissue culture plastic. We characterized the immunophenotype of cAM-MSCs by flow cytometry and measured cell proliferation by the cumulative population doubling level (CPDL). We performed differentiation studies for the detection of trilineage multipotent ability, under the appropriate culture conditions. Taken together, our results show that cAM-MSCs could be a rich source of stem cells in dogs. Furthermore, cAM-MSCs may be useful as a cell therapy application for veterinary regenerative medicine.
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