Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China | Zendy

Huili Zhu | Zendy; Jinfeng Zhao | Zendy; Rongjun Wang | Zendy; Longxian Zhang | Zendy

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Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China

Author(s) -

Huili Zhu,

Jinfeng Zhao,

Rongjun Wang,

Longxian Zhang

Publication year - 2012

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0043682

Subject(s) - subtyping , cryptosporidium , biology , ribosomal rna , virology , sequence analysis , polymerase chain reaction , gene , diarrhea , genetics , microbiology and biotechnology , medicine , feces , computer science , programming language

Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.

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