English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Gaf1 is the first GATA family zinc-finger transcription factor identified in  Schizosaccharomyces pombe . Here, we report that Gaf1 functions as a negatively acting transcription factor of  ste11 +  , delaying the entrance of cells exposed to transient nitrogen starvation into the meiotic cycle.  gaf1Δ  strains exhibited accelerated G 1 -arrest upon nitrogen starvation. Moreover,  gaf1Δ  mutation caused increased mating and sporulation frequency under both nitrogen-starved and unstarved conditions, while overexpression of  gaf1 +   led to a significant impairment of sporulation. By microarray analysis, we found that approximately 63% (116 genes) of the 183 genes up-regulated in unstarved  gaf1Δ  cells were nitrogen starvation-responsive genes, and furthermore that 25 genes among the genes up-regulated by  gaf1 Δ mutation are Ste11 targets ( e.g. ,  gpa1   + ,  ste4   + ,  spk1   + ,  ste11   + , and  mei2   + ). The phenotype caused by  gaf1Δ  mutation was masked by  ste11Δ  mutation, indicating that  ste11 +   is epistatic to  gaf1 +   with respect to sporulation efficiency, and accordingly that  gaf1 +   functions upstream of  ste11 +   in the signaling pathway governing sexual development.  gaf1Δ  strains showed accelerated  ste11 +   expression under nitrogen starvation and increased  ste11 +   expression even under normal conditions. Electrophoretic mobility shift assay analysis demonstrated that Gaf1 specifically binds to the canonical GATA motif (5′-HGATAR-3′) spanning from −371 to −366 in  ste11 +   promoter. Consequently, Gaf1 provides the prime example for negative regulation of  ste11 +   transcription through direct binding to a  cis -acting motif of its promoter.

The Fission Yeast GATA Factor, Gaf1, Modulates Sexual Development via Direct Down-Regulation of ste11+ Expression in Response to Nitrogen Starvation