3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of 18O-Trehalose in Mouse Sperm Nucleus | Zendy

C. Lechène | Zendy; Gloria Y. Lee | Zendy; J. Collin Poczatek | Zendy; Mehmet Toner | Zendy; John D. Biggers | Zendy

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3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of 18O-Trehalose in Mouse Sperm Nucleus

Author(s) -

C. Lechène,

Gloria Y. Lee,

J. Collin Poczatek,

Mehmet Toner,

John D. Biggers

Publication year - 2012

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0042267

Subject(s) - trehalose , sperm , desiccation , mass spectrometry , desiccation tolerance , mass spectrometry imaging , nucleus , isotope , chemistry , biology , microbiology and biotechnology , cryopreservation , biophysics , embryo , biochemistry , botany , chromatography , physics , quantum mechanics

The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.

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