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Background  To investigate the presence of metallo-β-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-β-lactamase gene  bla   NDM-1  in bacteria of food animal origin.    Methodology/Principal Findings  Gram-negative bacteria with low susceptibility to imipenem (MIC>8 µg/mL) were isolated from swab samples collected from 15 animal farms and one slaughterhouse in eastern China. These bacteria were selected for phenotypic and molecular detection of known MBL genes and antimicrobial susceptibility testing. For the  bla   NDM-1  positive isolate, conjugation and transformation experiments were carried out to assess plasmid transfer. Southern blotting was conducted to localize the  bla   NDM-1  genes, and DNA sequencing was performed to determine the sequences of  bla   NDM-1  and the flanking genes. In total, nine Gram-negative bacteria of four different species presented a MBL phenotype.  bla   NDM-1  was identified on a mobile plasmid named pAL-01 in an  Acinetobacter lwoffii  isolate of chicken origin. Transfer of pAL-01 from this isolate to  E. coli  J53 and JM109 resulted in resistance to multiple β-lactams. Sequence analysis revealed that the  bla   NDM-1  gene is attached to an intact insertion element IS Aba125 , whose right inverted repeat (IR-R) overlaps with the promoter sequence of  bla   NDM-1 . Thus, insertion of IS Aba125  likely enhances the expression of  bla   NDM-1 .    Conclusion  The identification of a  bla   NDM-1 - carrying strain of  A. lwoffii  in chickens suggests the potential for zoonotic transmission of  bla   NDM-1  and has important implications for food safety.

Identification of New Delhi Metallo-β-lactamase 1 in Acinetobacter lwoffii of Food Animal Origin