English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Much effort has focused recently on determining the mechanisms that control the allele-specific expression of genes subject to genomic imprinting, yet imprinting regulation is only one aspect of configuring appropriate expression of these genes. Imprinting control mechanisms must interact with those regulating the tissue-specific expression pattern of each imprinted gene in a cluster. Proper expression of the imprinted  Delta-like 1  ( Dlk1 ) -  Maternally expressed gene 3  ( Meg3 ) gene pair is required for normal fetal development in mammals, yet the mechanisms that control tissue-specific expression of these genes are unknown. We have used a combination of  in vivo  and  in vitro  expression assays to localize  cis -regulatory elements that may regulate  Dlk1  expression in the mouse embryo. A bacterial artificial chromosome transgene encompassing the  Dlk1  gene and 77 kb of flanking sequence conferred expression in most endogenous  Dlk1 -expressing tissues. In combination with previous transgenic data, these experiments localize the majority of  Dlk1 cis -regulatory elements to a 41 kb region upstream of the gene. Cross-species sequence conservation was used to further define potential regulatory elements, several of which functioned as enhancers in a luciferase expression assay. Two of these elements were able to drive expression of a  lacZ  reporter transgene in  Dlk1 -expressing tissues in the mouse embryo. The sequence proximal to  Dlk1  therefore contains at least two discrete regions that may regulate tissue-specificity of  Dlk1  expression.

Localizing Transcriptional Regulatory Elements at the Mouse Dlk1 Locus