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Yersinia outer protein J (YopJ) is a type III secretion system (T3SS) effector of pathogenic  Yersinia  ( Yersinia pestis ,  Yersinia enterocolitica  and  Yersinia pseudotuberculosis ) that is secreted into host cells. YopJ inhibits survival response pathways in macrophages, causing cell death. Allelic variation of YopJ is responsible for differential cytotoxicity in  Yersinia  strains. YopJ isoforms in  Y. enterocolitica  O:8 (YopP) and  Y. pestis  KIM (YopJ KIM ) strains have high cytotoxic activity. In addition, YopJ KIM -induced macrophage death is associated with caspase-1 activation and interleukin-1β (IL-1β secretion. Here, the mechanism of YopJ KIM -induced cell death, caspase-1 activation, and IL-1β secretion in primary murine macrophages was examined. Caspase-3/7 activity was low and the caspase-3 substrate poly (ADP-ribose) polymerase (PARP) was not cleaved in  Y. pestis  KIM5-infected macrophages. In addition, cytotoxicity and IL-1β secretion were not reduced in the presence of a caspase-8 inhibitor, or in B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax)/Bcl-2 homologous antagonist/killer (Bak) knockout macrophages, showing that YopJ KIM -mediated cell death and caspase-1 activation occur independent of mitochondrial-directed apoptosis. KIM5-infected macrophages released high mobility group protein B1 (HMGB1), a marker of necrosis, and microscopic analysis revealed that necrotic cells contained active caspase-1, indicating that caspase-1 activation is associated with necrosis. Inhibitor studies showed that receptor interacting protein 1 (RIP1) kinase and reactive oxygen species (ROS) were not required for cytotoxicity or IL-β release in KIM5-infected macrophages. IL-1β secretion was reduced in the presence of cathepsin B inhibitors, suggesting that activation of caspase-1 requires cathepsin B activity. Ectopically-expressed YopP caused higher cytotoxicity and secretion of IL-1β in  Y. pseudotuberculosis -infected macrophages than YopJ KIM . Wild-type and congenic caspase 1 knockout C57BL/6 mice were equally susceptible to lethal infection with  Y. pseudotuberculosis  ectopically expressing YopP. These data suggest that YopJ-induced caspase-1 activation in  Yersinia -infected macrophages is a downstream consequence of necrotic cell death and is dispensable for innate host resistance to a strain with enhanced cytotoxicity.

YopJ-Induced Caspase-1 Activation in Yersinia-Infected Macrophages: Independent of Apoptosis, Linked to Necrosis, Dispensable for Innate Host Defense