English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

In this work, we conducted functional analysis of  Arabidopsis HRS1  gene in order to provide new insights into the mechanisms governing seed germination. Compared with wild type (WT) control,  HRS1  knockout mutant ( hrs1-1 ) exhibited significant germination delays on either normal medium or those supplemented with abscisic acid (ABA) or sodium chloride (NaCl), with the magnitude of the delay being substantially larger on the latter media. The hypersensitivity of  hrs1-1  germination to ABA and NaCl required  ABI3 ,  ABI4  and  ABI5 , and was aggravated in the double mutant  hrs1-1abi1-2  and triple mutant  hrs1-1hab1-1abi1-2 , indicating that  HRS1  acts as a negative regulator of ABA signaling during seed germination. Consistent with this notion,  HRS1  expression was found in the embryo axis, and was regulated both temporally and spatially, during seed germination. Further analysis showed that the delay of  hrs1-1  germination under normal conditions was associated with reduction in the elongation of the cells located in the lower hypocotyl (LH) and transition zone (TZ) of embryo axis. Interestingly, the germination rate of  hrs1-1  was more severely reduced by the inhibitor of cell elongation, and more significantly decreased by the suppressors of plasmalemma H + -ATPase activity, than that of WT control. The plasmalemma H + -ATPase activity in the germinating seeds of  hrs1-1  was substantially lower than that exhibited by WT control, and fusicoccin, an activator of this pump, corrected the transient germination delay of  hrs1-1 . Together, our data suggest that  HRS1  may be needed for suppressing ABA signaling in germinating embryo axis, which promotes the timely germination of  Arabidopsis  seeds probably by facilitating the proper function of plasmalemma H + -ATPase and the efficient elongation of LH and TZ cells.

HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds