Open AccessSkip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of Alignments
Author(s) -
David Hysom,
Pejman NaraghiArani,
Maher Elsheikh,
A.C. Carrillo,
Peter L. Williams,
Shea N. Gardner
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0034560
Subject(s) - multiplex , taqman , computational biology , primer (cosmetics) , biology , primer binding site , oligonucleotide , virology , computer science , genetics , microbiology and biotechnology , bioinformatics , polymerase chain reaction , physics , dna , reverse transcriptase , gene , thermodynamics
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