Open AccessRUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1
Author(s) -
Lijie He,
Xiaodi Zhao,
Hanmin Wang,
Peng Zhang,
Changcun Guo,
Chen Huang,
Xiaowei Liu,
Fangfang Yao,
Yu Chen,
Weijuan Lou,
Shiren Sun,
Daiming Fan
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0032961
Subject(s) - cancer research , cyclin , cell growth , cyclin e1 , cell cycle , clear cell renal cell carcinoma , metastasis , cyclin d1 , cyclin a , chemistry , cell cycle checkpoint , cyclin e , microbiology and biotechnology , g1 phase , in vivo , growth inhibition , cyclin dependent kinase 2 , biology , cell , renal cell carcinoma , medicine , pathology , cancer , biochemistry , genetics
Here we presented that the expression of RUNX3 was significantly decreased in 75 cases of clear cell renal cell carcinoma (CCRCC) tissues (p<0.05). Enforced RUNX3 expression mediated 786-O cells to exhibit inhibition of growth, G1 cell-cycle arrest and metastasis in vitro , and to lost tumorigenicity in nude mouse model in vivo . RUNX3-induced growth suppression was found partially to regulate various proteins, including inhibition of cyclinD1, cyclinE, cdk2, cdk4 and p-Rb, but increase of p27 Kip1 , Rb and TIMP-1. Therefore, RUNX3 had the function of inhibiting the proliferative and metastatic abilities of CCRCC cells by regulating cyclins and TIMP1.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom