SPECT/CT Imaging of hNIS -Expression after Intravenous Delivery of an Oncolytic Adenovirus and 131I | Zendy

Maria Rajecki | Zendy; Mirkka Sarparanta | Zendy; Tanja Hakkarainen | Zendy; Mikko Tenhunen | Zendy; Iulia Diaconu | Zendy; Venla Kuhmonen | Zendy; Kalevi Kairemo | Zendy; Anna Kanerva | Zendy; Anu J. Airaksinen | Zendy; Akseli Hemminki | Zendy

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SPECT/CT Imaging of hNIS -Expression after Intravenous Delivery of an Oncolytic Adenovirus and 131I

Author(s) -

Maria Rajecki,

Mirkka Sarparanta,

Tanja Hakkarainen,

Mikko Tenhunen,

Iulia Diaconu,

Venla Kuhmonen,

Kalevi Kairemo,

Anna Kanerva,

Anu J. Airaksinen,

Akseli Hemminki

Publication year - 2012

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0032871

Subject(s) - oncolytic virus , sodium iodide symporter , oncolytic adenovirus , telomerase reverse transcriptase , genetic enhancement , cancer research , in vivo , adenoviridae , medicine , virotherapy , transgene , gene delivery , biology , telomerase , gene , symporter , tumor cells , biochemistry , microbiology and biotechnology , transporter

Oncolytic adenoviruses can be engineered for better tumor selectivity, gene delivery and be armed for imaging and concentrating radionuclides into tumors for synergistic oncolysis. We constructed Ad5/3-hTERT-hNIS where replication is controlled by hTERT-promoter. Ad5/3-hTERT-hNIS expresses hNIS for imaging of transgene expression and for treatment of infected tumors by radioiodine. Ad5/3-hTERT-hNIS efficiently killed prostate cancer cells and induced iodine uptake in vitro and in vivo after intratumoral virus administration. Survival of mice treated with intravenous Ad5/3-hTERT-hNIS significantly prolonged survival over mock or radioiodine only but the combination of virus with radioiodine was not more effective than virus alone. Temporal and spatial changes in hNIS-expression during therapy were detected with SPECT, demonstrating feasibility of evaluation of the combination therapy with hNIS-expressing adenoviruses and radioiodide.

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