Open AccessHIV-1 Inhibits Phagocytosis and Inflammatory Cytokine Responses of Human Monocyte-Derived Macrophages to P. falciparum Infected Erythrocytes
Author(s) -
Louise E. Ludlow,
Jingling Zhou,
Emma Tippett,
WanJung Cheng,
Wina Hasang,
Stephen J. Rogerson,
Anthony Jaworowski
Publication year - 2012
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0032102
Subject(s) - phagocytosis , proinflammatory cytokine , plasmodium falciparum , cytokine , monocyte , immunology , malaria , macrophage , interleukin 10 , secretion , microbiology and biotechnology , biology , human immunodeficiency virus (hiv) , inflammation , in vitro , endocrinology , biochemistry
HIV-1 infection increases the risk and severity of malaria by poorly defined mechanisms. We investigated the effect of HIV-1 Ba-L infection of monocyte-derived macrophages (MDM) on phagocytosis of opsonised P. falciparum infected erythrocytes (IE) and subsequent proinflammatory cytokine secretion. Compared to mock-infected MDM, HIV-1 infection significantly inhibited phagocytosis of IE (median (IQR) (10 (0–28) versus (34 (27–108); IE internalised/100 MDM; p = 0.001) and decreased secretion of IL-6 (1,116 (352–3,387) versus 1,552 (889–6,331); pg/mL; p = 0.0078) and IL-1β (16 (7–21) versus 33 (27–65); pg/mL; p = 0.0078). Thus inadequate phagocytosis and cytokine production may contribute to impaired control of malaria in HIV-1 infected individuals.
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