Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain | Zendy

Partho Halder | Zendy; YiChun Chen | Zendy; Janine Brauckhoff | Zendy; Alois Hofbauer | Zendy; MarieChristine Dabauvalle | Zendy; Urs Lewandrowski | Zendy; Christiane Winkler | Zendy; Albert Sickmann | Zendy; Erich Buchner | Zendy

Open Access

Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain

Author(s) -

Partho Halder,

YiChun Chen,

Janine Brauckhoff,

Alois Hofbauer,

MarieChristine Dabauvalle,

Urs Lewandrowski,

Christiane Winkler,

Albert Sickmann,

Erich Buchner

Publication year - 2011

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0029352

Subject(s) - monoclonal antibody , microbiology and biotechnology , antigen , clone (java method) , biology , immunohistochemistry , antibody , mutant , western blot , drosophila (subgenus) , gene , genetics , immunology

The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.

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