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Human Elongator complex, which plays a key role in transcript elongation  in vitro  assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization  in vivo . We studied the effect of down-regulation of the human elongation protein 3 (h ELP3 ) on the expression of  HSP70  through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed h ELP3  expression, and substantially reduced expression of  HSP70  mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of  HSPA1A  in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of  HSP70  ( SSA3 ) gene transcription, but also directly regulates the transcription of  SSA3 . On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of  HSP70  gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of h ELP3  in human cells.

hElp3 Directly Modulates the Expression of HSP70 Gene in HeLa Cells via HAT Activity