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Recent advancements in genomics provide new tools for evolutionary ecological research. The paper wasp genus  Polistes  is a model for social insect evolution and behavioral ecology. We developed RNA interference (RNAi)-mediated gene silencing to explore proposed connections between expression of hexameric storage proteins and worker vs. gyne (potential future foundress) castes in naturally-founded colonies of  P. metricus . We extended four fragments of putative hexamerin-encoding  P. metricus  transcripts acquired from a previous study and fully sequenced a gene that encodes Hexamerin 2, one of two proposed hexameric storage proteins of  P. metricus . MALDI-TOF/TOF, LC-MSMS, deglycosylation, and detection of phosphorylation assays showed that the two putative hexamerins diverge in peptide sequence and biochemistry. We targeted the  hexamerin 2  gene in 5 th  (last)-instar larvae by feeding RNAi-inducing double-stranded  hexamerin 2  RNA directly to larvae in naturally-founded colonies in the field. Larval development and adult traits were not significantly altered in  hexamerin 2  knockdowns, but there were suggestive trends toward increased developmental time and less developed ovaries, which are gyne characteristics. By demonstrating how data acquisition from 454/Roche pyrosequencing can be combined with biochemical and proteomics assays and how RNAi can be deployed successfully in field experiments on  Polistes , our results pave the way for functional genomic research that can contribute significantly to learning the interactions of environment, development, and the roles they play in paper wasp evolution and behavioral ecology.

Development of an RNA Interference Tool, Characterization of Its Target, and an Ecological Test of Caste Differentiation in the Eusocial Wasp Polistes